Journal: Toxins

Article Title: Effect of Zinc on Microcystis aeruginosa UTEX LB 2385 and Its Toxin Production

doi: 10.3390/toxins12020092

Figure Lengend Snippet: ELISA quantitative analysis of intracellular and extracellular MC-LR (µg/L) equivalents within Microcystis aeruginosa UTEX LB 2385 treated with 0, 0.1, 0.25, and 0.5 mg/L ZnCl 2 at Days: One, 5, 8, 12, 15. Extracellular MC-LR equivalents (µg/L) of 0.1 mg/L ZnCl 2 cells showed a 18% and 23% increase for Days 8 and 12 compared to control; 0.25 and 0.5 mg/L ZnCl 2 treated cells showed a ~40% and 33% increase of extracellular MC-LR, respectively, as early as day 5. The 0.25 mg/L ZnCl 2 extracellular MC-LR (µg/L) equivalents decreased and increased from Days 8 to 15, and 0.5 mg/L ZnCl 2 intracellular MC-LR (µg/L) intracellular measurements decreased and increased from this same time point. All samples were performed as duplicate replicates.

Article Snippet: ELISA quantitation for intracellular and extracellular microcystin-LR equivalents was performed using a Microcystin-LR ELISA kit (colorimetric) (Abnova, Taipei, Taiwan) as per manufacturer specifications.

Techniques: Enzyme-linked Immunosorbent Assay, Control